Coding
T5OH

Part:BBa_K4201007

Designed by: Maya Li Nelson   Group: iGEM22_CU-Boulder   (2022-10-01)


T5αOH

The Taxane 5α-hydroxylase (T5αOH) naturally occuring in Yew trees metabolizes Taxadiene, the precursor molecule to Paclitaxel, into Taxadiene-5α-ol1. T5αOH is the second step in the synthesis pathway of paclitaxel1. Successful integration of this enzyme could help further the progress of the biosynthesis of paclitaxel.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 184
    Illegal BglII site found at 800
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 184
    Illegal NgoMIV site found at 333
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1517


[edit]
Categories
Parameters
None